Analysis of genetic diversity among different tomato genotypes using ISSR DNA marker

Abstract

Inter simple sequence repeat (ISSR) markers were used for variation analysis among 41 tomato accessions. A total of 50 scorable bands were obtained, where 32 were polymorphic, representing 63.3% of all the amplified loci. Polymorphism percentage ranged from 50 to 90% and an average number of polymorphic bands of 4.0 were observed. An average genetic diversity index was 0.61. Primer UBC860 and UBC825 generated the greatest diversity index with a value of 0.89 and 0.85 respectively. The smallest diversity identified by primer UBC808, with an index of 0.34. The genetic similarity among studied genotypes ranged from 0.52 to 0.98. The cluster analysis based on Jaccard’s similarity coefficient divided genotypes into 6 distinct clusters on a value of 0.74. The lowest genetic distance was found between ‘Gronastiy’ and ‘AG1224’ (0.52), ‘Orange’ and ‘AG1224’ (0.54), and ‘Evgeniya’ and ‘AG1224’ (0.55) accessions. The highest similarity of 0.98 was determined between ‘Zafar’ and ‘Azerbaijan-94’, ‘Khachmaz-1’ and ‘Azerbaijan-94’, ‘Khachmaz-1’ and ‘Severyanka’, and ‘Shakar’ and ‘Absheron-1’ accessions.