DSpace Collection:
http://hdl.handle.net/20.500.12323/5694
2024-03-29T02:21:47ZVerification of taxonomic relationships within the genus Secale (Poaceae: Pooideae: Triticeae) based on multiple molecular methods
http://hdl.handle.net/20.500.12323/5700
Title: Verification of taxonomic relationships within the genus Secale (Poaceae: Pooideae: Triticeae) based on multiple molecular methods
Authors: Targońska-Karasek, Małgorzata; Bolibok-Brągoszewska, Hanna; Oleniecki, Tymoteusz; Sharifova, Saida; Kopania, Marta; Rakoczy-Trojanowska, Monika
Abstract: This study aimed to verify the taxonomic relationships within the genus Secale. The plant material included 16 wild rye accessions
from four species. Two approaches were applied: 1) whole genome scanning using three molecular marker systems:
diversity arrays technology sequencing, simple sequence repeats and sequence-specific amplification polymorphism; and
2) characterisation based on polymorphisms within the sequences of two genes involved in benzoxazinoid biosynthesis:
ScBx1 and ScBx5. Bayesian and neighbour-joining clustering and principal coordinate analysis were applied to illustrate
relationships among species and accessions of Secale based on genetic distance (GD) matrices. Pearson’s correlation analysis
between GD matrices was conducted. Clustering of Secale accessions revealed that S. sylvestre samples were the most
divergent. The remaining accessions formed two clusters. One of them comprised S. strictum accessions while the second
cluster consisted of subspecies of S. cereale, the species S. vavilovii and S. strictum subsp. ciliatoglume.2018-01-01T00:00:00ZISSR Analysis of Variability of Cultivated Form and Varieties of Pomegranate (Punica granatum L.) from Azerbaijan
http://hdl.handle.net/20.500.12323/5699
Title: ISSR Analysis of Variability of Cultivated Form and Varieties of Pomegranate (Punica granatum L.) from Azerbaijan
Authors: Hajiyeva, S. V.; Akparov, Z. I.; Mustafayeva, Z. P.; Hasanov, N. A.; Hajiyev, E. S.; Mammadov, A. T.; Izzatullayeva, V. I.; Babayeva, S. M.; Sharifova, S. S.; Mammadov, A. M.; Abbasov, M. A.
Abstract: The article presents the results of a study of genetic polymorphism for the first time carried out on
pomegranate varieties and forms of Azerbaijan origin using molecular markers. In total, 102 PCR fragments
were identified, of which 80 were polymorphic. The high level of polymorphism (75.5%) and the rich genetic
diversity were identified among the studied pomegranate collection. As a result of data analysis and on the
basis of the values of the basic parameters (PIC, EMR, MI, RP, MRP) determining informativeness of
markers, all 14 ISSR primers were suitable for genotyping pomegranate accessions. The most effective markers
(UBC808, UBC811, UBC834, and UBC840) were identified among the set of primers tested. A dendrogram
was constructed on the basis of the data obtained, which made it possible to group genotypes into 16
major clusters. The genetic similarity index ranged from 0.032 to 0.94. The study of the genetic relationship
of different pomegranate varieties confirms the effectiveness of the ISSR method, which makes it possible to
determine the level of genetic diversity, as well as to establish the relationship among the studied pomegranate
accessions.2018-01-01T00:00:00ZIn silico analysis on frequency and types of simple sequence repeats in expressed sequence tags (ESTs) of different tomato species
http://hdl.handle.net/20.500.12323/5698
Title: In silico analysis on frequency and types of simple sequence repeats in expressed sequence tags (ESTs) of different tomato species
Authors: Sharifova, S.S.; Aliyev, R.T.; Shahmuradova, I.A.
Abstract: The present study focuses on computational mining and survey of the frequency and types of simplesequence repeats in Expressed Sequence Tags (ESTs) of three different tomato species. A thousand ESTs sequences from each of S.lycopersicum L., S.pennellii Corell. and S.habrochaites S.Knapp & D.M.Spooner were retrieved from the National Center for Biotechnology Information (NCBI) databaseand searched for the presence of simple sequence repeat (SSRs) motifs. A total of 35 EST-derived SSRs within 32 unigens of S.lycopersicum, 51 SSRs within 46 unigens of S.pennellii and 64 SSRs in 55unigens of S.habrochaites were identified. Trinucleotide repeats were the most abundant class of microsatellites in all the examined species (54%, 39% and 45% respectively). The hexanucleotide repeats were the second most frequent EST-SSRs in cultivated tomato (26%) and s. pennellii (27%), whereas trinucleotide motifs were followed by pentanucleotides in S. habrochaites (28%). The AGC/CTG motif was the most frequent among all types of repeats identified in S. lycopersicum ESTs, whereas the AAT/ATT and AT/AT were the most abundant in S. pennellii and AAATT/AATTT, ACC/GGT, AAT/ATT, ATC/ATG and AT/AT in S. habrochaites respectively.2016-01-01T00:00:00ZInvestigation of genetic diversity of wheat genotypes (Triticum aestivum L.) using microsatellite markers
http://hdl.handle.net/20.500.12323/5697
Title: Investigation of genetic diversity of wheat genotypes (Triticum aestivum L.) using microsatellite markers
Authors: Mursalova, J.M.; Akparov, Z.I.; Sharifova, S.S.; Ojaghi, J.M.; Eldarov, M.E.; Morgounov, A.I.; Trojanowska, M.R.
Abstract: The study was devoted to evaluate genetic diversity of 48 winter wheat genotypes (varieties and advanced lines) with different origins using simple sequence repeat (SSR) markers/ Out of 20 SSR primer pairs used for assessing the genetic diversity, 19 primer pairs were polymorphic. Microsatellite PIC values ranged from 0.26 to 0.93 with an average value of 0.740. The cluster analysis based on Nei genetic distance indices divided genotypes into four main groups/ The Xwmc24, Xwmc169, Xgpw322, Xgwm297 and Xbarc267 primers amplified high number of alleles, showing relatively high level of polymorphism and evaluated as efficient primers for genetic diversity analysis of wheat populations.2015-01-01T00:00:00Z